Journal: Cell Death & Disease

Article Title: TNFα and IL-1β modify the miRNA cargo of astrocyte shed extracellular vesicles to regulate neurotrophic signaling in neurons

doi: 10.1038/s41419-018-0369-4

Figure Lengend Snippet: a Representative recording of population spikes (each purple vertical line), and bursts (purple clusters with >4 spikes/s) from an individual electrode of a multichannel electrode array. Representative tracings show averages of spikes/second/electrode, and associated scatter plots show quantitation of spike and burst rates for ( b – e ) Control, ( f – i ) ADEV-IL-1β (particle dose of 50 ADEVs/cell), ( j – m ) ADEV-IL-1β+ Scrambled oligonucleotide (Scr In, 20 pmole), and ( n – q ) ADEV-IL-1β+ oligonucleotide inhibitors for miR-125 and miR-16 (Combined In, 20 pmole each). Data are mean ± SEM. Paired t -tests were performed to compare spike and burst rate of each electrode before and after treatment. *** p < 0.001 increased compared to baseline and ### p < 0.001 decreased compared to baseline

Article Snippet: In brief, hippocampal tissues were separately dissociated by gentle trituration in a calcium-free Hank’s balanced salt solution and centrifuged at 1000× g . Cells were resuspended in Neurobasal media (Gibco) containing B27 supplement (Thermo Fisher Scientific), 1% antibiotic/antimitotic solution (104 Unit of penicillin G/ml, 10 mg streptomycin/ml and 25 μg amphotericin B/ml) (Sigma), and plated at a density of 40,000 cells/ml in 96-well plates (Corning) coated with polyethyleneimine (Sigma) or 160,000 cells/ml in multichannel electrode array chambers (Harvard Apparatus) coated with polyethyleneimine (Sigma) and laminin (Sigma).

Techniques: Quantitation Assay